A Physical Sciences Network Characterization of Non-tumorigenic and Metastatic Cells. Sci Rep. 2013
A physical sciences network characterization of non-tumorigenic and metastatic cells.
‘Isotropic 3D Nuclear Morphometry of normal, fibrocystic and malignant breast epithelial cells reveals novel structural alterations’ with Vivek Nandakumar, Laimonas Kelbauskas, Kathryn Hernandez, Kelly Lintecum, Patti Senechal, Kimberly Bussey, Roger Johnson and Deirdre Meldrum, PLoS ONE (2012).
Isotropic 3D Nuclear Morphometry of normal, fibrocystic and malignant breast epithelial cells reveals novel structural alterations PDF
Vivek Nandakumar, Deirdre Meldrum and Roger Johnson publish “Quantitative Characterization of Preneoplastic Progression Using Single-Cell Computed Tomography and Three-Dimensional Karyometry”
Quantitative characterization of preneoplastic progression using single-cell computed tomography and three-dimensional karyometry.
A hallmark of cancer is that the cell nucleus changes shape and size as the cell transforms from healthy to premalignant to malignant, and cancer researchers suspect that an accurate method of quantifying those changes could serve as an early diagnostic test for cancer. Now, a team of investigators at Arizona State University have given cancer researchers an imaging tool that should enable them to determine if that suspicion is correct.
Deirdre Meldrum and Roger Johnson, both members of the Arizona State University Physical Sciences-Oncology Center (PS-OC), led the team that used high-resolution optical absorption tomographic imaging and mathematical reconstruction to create detailed three-dimensional images of the cell nucleus. They then took those images and used an automated analytical technique they developed to compute 41 quantitative descriptions of a cell’s nuclear structure. The investigators also developed mathematical tools to quantify the spatial distribution of DNA within the cell nucleus from the reconstructed optical images.
Using these techniques, the Arizona PS-OC team compared the three-dimensional architecture of malignant, premalignant, and malignant esophageal epithelial cells and identified quantitative differences in cell shape among the three cell types. Moreover, the researchers were able to clearly distinguish between the three different types of cells using those quantitative measures. The researchers published the results of their study in the journal Cytometry Part A.
This work, which is detailed in a paper titled, “Quantitative Characterization of Preneoplastic Progression Using Single-Cell Computed Tomography and Three-Dimensional Karyometry,” was supported by the National Cancer Institute’s Physical Sciences-Oncology Centers program that aims to foster the development of innovative ideas and new fields of study based on knowledge of the biological and physical laws and principles that define both normal and tumor systems. An abstract of this paper is available at the journal’s Web site.